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C4982

Sigma-Aldrich

Cytochrome P450 human

3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

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Enzyme Commission number:
MDL number:
NACRES:
NA.47

biological source

human

Quality Level

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

form

solution

mol wt

45-60 kDa

packaging

vial of ~0.5 nmol
vial of ≥25 units

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP3A4(1576)

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This Item
C3735C5235C3860
Sigma-Aldrich

C4982

Cytochrome P450 human

Sigma-Aldrich

C3735

Cytochrome P450 human

Sigma-Aldrich

C5235

Cytochrome P450 human

Sigma-Aldrich

C3860

Cytochrome P450 human

biological source

human

biological source

human

biological source

human

biological source

human

form

solution

form

solution

form

solution

form

solution

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Biochem/physiol Actions

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Other Notes

Microsomes containing recombinant human CYP3A4 and recombinant rabbit NADPH-P450 reductase

Unit Definition

One unit will convert 1 nanomole of testosterone to 6β-hydroxytestosterone per minute at pH 7.4 at 37 °C.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yasuaki Kabe et al.
Cancers, 13(13) (2021-07-03)
Progesterone receptor membrane component 1 (PGRMC1) is highly expressed in various cancer cells and contributes to tumor progression. We have previously shown that PGRMC1 forms a unique heme-stacking functional dimer to enhance EGF receptor (EGFR) activity required for cancer proliferation
Andreia Palmeira et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 15(1), 31-45 (2012-03-01)
Aminated thioxanthones have recently been described as dual-acting agents: growth inhibitors of leukemia cell lines and P-glycoprotein (P-gp) inhibitors. To evaluate the selectivity profile of thioxanthones as inhibitors of multidrug resistance (MDR), their interaction with other ABC transporters, which were
Yasuaki Kabe et al.
Nature communications, 7, 11030-11030 (2016-03-19)
Progesterone-receptor membrane component 1 (PGRMC1/Sigma-2 receptor) is a haem-containing protein that interacts with epidermal growth factor receptor (EGFR) and cytochromes P450 to regulate cancer proliferation and chemoresistance; its structural basis remains unknown. Here crystallographic analyses of the PGRMC1 cytosolic domain
Xiangrong Zhang et al.
PloS one, 9(4), e94962-e94962 (2014-04-17)
The present study characterized in vitro metabolites of 20(R)-25-methoxyl-dammarane-3β, 12β, 20-triol (20(R)-25-OCH3-PPD) in mouse, rat, dog, monkey and human liver microsomes. 20(R)-25-OCH3-PPD was incubated with liver microsomes in the presence of NADPH. The reaction mixtures and the metabolites were identified
Dongju Lin et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(10), 1727-1736 (2014-07-16)
Diosbulbin B (DIOB), a furan-containing diterpenoid lactone, is the most abundant component of Dioscorea bulbifera L. (DB), a traditional Chinese medicine herb. Administration of purified DIOB or DB extracts has been reported to cause liver injury in animals. The mechanisms

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