C8051
Collagenase from Clostridium histolyticum
Sigma Blend Type H, ≥1.0 FALGPA units/mg solid
Recommended Products
Quality Level
form
powder
caseinase activity
≥10 units/mg solid
specific activity
≥1.0 FALGPA units/mg solid
mol wt
68-130 kDa
storage temp.
−20°C
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Related Categories
Application
- to harvest osteosarcoma 143B cells from collagen scaffold
- in tissue digestion of islets and carotid artery
- in the digestion of chorionic villi
- in enzymatic dissociation for ATP-based tumour chemosensitivity assay (ATP-TCA) in solid tumors
Biochem/physiol Actions
Unit Definition
Substrate
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Protocols
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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