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C8571

Sigma-Aldrich

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

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Synonym(s):
Cathepsin B1
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.32

biological source

human liver

Quality Level

form

buffered aqueous solution

specific activity

≥1,500 units/mg protein (E1%/280)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSB(1508)

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C8571

Cathepsin B from human liver

vibrant-m

C6286

Cathepsin B from bovine spleen

vibrant-m

C6854

Cathepsin L from human liver

vibrant-m

C0150

Cathepsin B from human placenta

Gene Information

human ... CTSB(1508)

Gene Information

cow ... CTSB(281105)

Gene Information

human ... CTSL1(1514)

Gene Information

human ... CTSB(1508)

biological source

human liver

biological source

bovine spleen

biological source

human liver

biological source

-

UniProt accession no.

P07858

UniProt accession no.

P07688

UniProt accession no.

P07711

UniProt accession no.

P07858

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:
  • as a positive control in the screening Cathepsin B inhibitor assay
  • in wall extension (expansin activity) assay
  • in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
Wu X, et al.
Hepatology, 47(6), 1905-1915 (2008)
Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts
Roberg K, et al.
The American Journal of Pathology, 161(1), 89-96 (2002)

Articles

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

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