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C9301

Sigma-Aldrich

Collagen from chicken sternal cartilage

Type II (Miller), powder, BioReagent, suitable for cell culture

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

chicken (Sternal cartilage)

Quality Level

type

Type II (Miller)

product line

BioReagent

form

powder

packaging

glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg

concentration

60-80% (biuret)

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

UniProt accession no.

binding specificity

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

chicken ... COL2A1(395069)

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This Item
C9791C9879C0543
vibrant-m

C9791

Collagen from calf skin

biological source

chicken (Sternal cartilage)

biological source

bovine (calf) skin

biological source

bovine Achilles tendon

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, activity assay: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

UniProt accession no.

P02460

UniProt accession no.

P02453

UniProt accession no.

P02459

UniProt accession no.

B1AVK5, P02463, P08122, Q63ZW6, Q9QZS0

binding specificity

Peptide Source: Fibrinogen

binding specificity

Peptide Source: Fibronectin
, Peptide Source: Laminin

binding specificity

-

binding specificity

-

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used:
  • in enzyme–linked immunosorbent assay (ELISA).
  • chondrocyte-mediated tissue production in vitro.
  • induction, treatment and assessment of collagenα induced arthritis (CIA).
  • cell proliferation assay.
  • as a coating for cell culture surfaces.

Biochem/physiol Actions

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Features and Benefits

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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