C9407
Collagenase from Clostridium histolyticum
powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture
Synonym(s):
Clostridiopeptidase A
About This Item
Recommended Products
biological source
Clostridium histolyticum
Quality Level
form
powder
specific activity
≥800 units/mg solid
mol wt
68-130 kDa
packaging
pkg of 1 μmol
technique(s)
cell culture | mammalian: suitable
pH
7.4
storage temp.
−20°C
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General description
Application
- of pancreatic samples for islets isolation
- of pulmonary artery samples for pulmonary vascular smooth muscle cells (PVSMCs) isolation
- of cartilage samples for chondrocytes isolation
Biochem/physiol Actions
Caution
Unit Definition
Preparation Note
Substrate
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Articles
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Protocols
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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