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C9422

Sigma-Aldrich

Cellulase from Trichoderma viride

greener alternative

crude powder, 3-10 units/mg solid

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase

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About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
12352204

form

crude powder

specific activity

3-10 units/mg solid

greener alternative product characteristics

Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase, or cellulase, has been used in a study to examine supra-molecular structural features of cellulose during enzymatic hydrolysis using CP/MAS 13C NMR spectra in combination with line-fitting analysis.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Substrate

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Does Product C9422, Cellulase from Trichoderma viride, contain any sugars as a stabilizer?

    Product C9422, Cellulase from Trichoderma viride,contains 10 - 20% dextrin as a stabilizer.

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Jack P Wang et al.
Nature communications, 9(1), 1579-1579 (2018-04-22)
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa
Yunqiao Pu et al.
Carbohydrate research, 341(5), 591-597 (2006-01-31)
Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-beta-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS 13C NMR spectra in combination with line-fitting analysis. Different types of
Eliana N Fissore et al.
Journal of agricultural and food chemistry, 58(6), 3793-3800 (2010-02-25)
Chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. These fractions were obtained through acid hydrolysis of butternut (Cucurbita moschata Duch ex Poiret) and red beet (Beta vulgaris L. var. conditiva) cell wall enriched powders. Hydrolysis
Laura Sutarlie et al.
Journal of colloid and interface science, 411, 76-81 (2013-10-12)
Cellulase is an important enzyme for hydrolyzing cellulose to form glucose. To recycle cellulase after the reaction, cellulase is often immobilized on solid supports but its activity is also compromised. In this study, we show a new hybrid cellulase aggregate
Mark A Currie et al.
The Journal of biological chemistry, 288(11), 7978-7985 (2013-01-24)
Clostridium thermocellum produces the prototypical cellulosome, a large multienzyme complex that efficiently hydrolyzes plant cell wall polysaccharides into fermentable sugars. This ability has garnered great interest in its potential application in biofuel production. The core non-catalytic scaffoldin subunit, CipA, bears
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