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D0426

Sigma-Aldrich

SIGMAFAST DAB with Metal Enhancer

tablet

Synonym(s):

3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

form

tablet

Quality Level

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

General description

SIGMA FAST DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Application

SIGMAFAST DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocols

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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