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D0550

Sigma-Aldrich

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

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Synonym(s):
3,5-Dinitro-2-hydroxybenzoic acid, DNS
Linear Formula:
(O2N)2C6H2-2-(OH)CO2H
CAS Number:
Molecular Weight:
228.12
Beilstein/REAXYS Number:
2220661
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

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This Item
12884827301D131
3,5-Dinitrosalicylic acid used in colorimetric determination of reducing sugars

D0550

3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid 98%

128848

3,5-Dinitrosalicylic acid

Salicylic acid meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (calc. to the dried substance)

27301

Salicylic acid

3,5-Dinitrocatechol solid

D131

3,5-Dinitrocatechol

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

H2O: 50 mg/mL

solubility

-

solubility

-

solubility

H2O: slightly soluble 0.17 mg/mL, 45% (w/v) aq 2-hydroxypropyl-β-cyclodextrin: 2.8 mg/mL, 0.1 M HCl: slightly soluble, DMSO: soluble, aqueous buffer pH > 5: soluble, ethanol: soluble

form

powder

form

powder

form

-

form

solid

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

2-8°C

mp

168-172 °C (lit.)

mp

168-172 °C (lit.)

mp

158-161 °C (lit.)

mp

168-170  °C

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Use of dinitrosalicylic acid reagent for determination of reducing sugar.
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