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D6429

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
DME, DMEM

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
D6546D1145FG0435
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

components

phenol red: yes
glucose: high
stable glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium - high glucose has been used for cell culture.
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Mouse sphingosine kinase isoforms SPHK1a and SPHK1b differ in enzymatic traits including stability, localization, modification, and oligomerization.
Kihara A, et al.
The Journal of Biological Chemistry, 281(7), 4532-4539 (2006)
Neuraminidase receptor binding variants of human influenza A(H3N2) viruses resulting from substitution of aspartic acid 151 in the catalytic site: a role in virus attachment?
Lin YP, et al.
Journal of Virology, 84(13), 6769-6781 (2010)
Kazuhiro Ikeda et al.
Scientific reports, 7(1), 2850-2850 (2017-06-08)
Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed.
Kenta Imai et al.
Journal of cell science, 129(20), 3781-3791 (2016-09-03)
Autophagy is an intracellular degradation pathway conserved in eukaryotes. Among core autophagy-related (Atg) proteins, mammalian Atg9A is the sole multi-spanning transmembrane protein, and both of its N- and C-terminal domains are exposed to the cytoplasm. It is known that Atg9A
Mikiko Fukuda et al.
The Journal of reproduction and development, 62(1), 121-125 (2015-11-26)
Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for investigating the function of specific genes in vivo. Although the protocol for gene targeting via homologous recombination (HR) in ESCs is already well established, the

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Cell culture protocol for passaging and splitting suspension cell lines

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