D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: glucose: 3.15 g/L; NaHCO₃: yes; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: 0.055 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
• to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
• to culture mouse squamous cell carcinoma (SCCVII) tumor cells

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable