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E2156

Sigma-Aldrich

Anti-EGF Receptor antibody, Mouse monoclonal

clone 225, purified from hybridoma cell culture

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Synonym(s):
Anti-EGFR, Anti-Epidermal Growth Factor Receptor
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

225, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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biological source

mouse

biological source

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biological source

mouse

biological source

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antibody form

purified immunoglobulin

antibody form

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ascites fluid

antibody form

purified immunoglobulin

clone

225, monoclonal

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F4, monoclonal

clone

225, monoclonal

conjugate

unconjugated

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species reactivity

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species reactivity

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species reactivity

human

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General description

Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.

Immunogen

partially purified EGF receptors from human A-431 cells.

Application

Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.
Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).

Biochem/physiol Actions

EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.
EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .

Physical form

0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Legal Information

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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