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E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
EDTA solution
Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
MDL number:
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

solution

concentration

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.1-7.7

shipped in

wet ice

storage temp.

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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1 of 4

This Item
E988403620E6758
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder

Sigma-Aldrich

E9884

Ethylenediaminetetraacetic acid

Essential Grade
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture

Sigma-Aldrich

E6758

Ethylenediaminetetraacetic acid

Essential+ Grade
form

solution

form

powder

form

powder

form

crystalline

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

impurities

-

impurities

-

pH

7.1-7.7

pH

2.5 (23 °C, 10 g/L)

pH

-

pH

2.5 (23 °C, 10 g/L)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ex vivo Lung bioengineering
Lung Stem Cells in the Epithelium and Vasculature, 151-151 (2015)
The genotoxicity of physiological concentrations of chromium (Cr (III) and Cr (VI)) and cobalt (Co (II)): an in vitro study
Figgitt M, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 688(1-2), 53-61 (2010)
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Wang Y, et al.
Stem Cell Research & Therapy, 8(1), 189-189 (2017)
Cerium fluoride nanoparticles protect cells against oxidative stress.
Shcherbakov AB, et al.
Materials Science and Engineering, C, 50, 151-159 (2015)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)

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