ED4SS
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate
99.0-102.0% (titration)
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Quality Level
assay
99.0-102.0% (titration)
form
crystalline
reaction suitability
reagent type: chelator
solubility
H2O: soluble, clear, colorless
SMILES string
O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4
InChI key
KSYNLCYTMRMCGG-UHFFFAOYSA-J
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General description
Application
- in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
- as a component of NH4Cl lysis buffer for erythrocyte lysis and bulk characterization
- for the isolation of crypts from the connective tissues of mice
Biochem/physiol Actions
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2
target_organs
Respiratory Tract
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Articles
Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.
Protocols
Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
This procedure may be used for all Ficin products.
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