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F0635

Sigma-Aldrich

Fibronectin rat plasma

powder, BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
EC Number:
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

biological source

rat plasma

Quality Level

sterility

sterile

product line

BioReagent

form

powder

packaging

pkg of 0.5 mg
pkg of 1 mg
pkg of 2 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)

UniProt accession no.

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Fn1(25661)

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This Item
F2518F1056F2006
vibrant-m

F0635

Fibronectin rat plasma

vibrant-m

F1056

Fibronectin human plasma

vibrant-m

F2006

Fibronectin human plasma

sterility

sterile

sterility

-

sterility

sterile

sterility

sterile

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

rat plasma

biological source

human foreskin fibroblasts

biological source

human plasma

biological source

human plasma

Gene Information

rat ... Fn1(25661)

Gene Information

human ... FN1(2335)

Gene Information

human ... FN1(2335)

Gene Information

human ... FN1(2335)

solubility

H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

solubility

H2O: 1 mg/mL at 37 °C

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

General description

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

Application

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.

Biochem/physiol Actions

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Analysis Note

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Neuronal nitric oxide synthase immunohistochemistry and 4, 5-diaminofluorescein diacetate: tools for nitric oxide research.
Kasim N, et al.
Journal of Neuroscience Methods, 112(1), 1-8 (2001)
Yang Gao et al.
Molecules (Basel, Switzerland), 27(9) (2022-05-15)
There is an urgent need to develop new effective therapies for HCC. Our previous study identified ULK1 as the potential target for HCC therapy and screened the compound XST-14 as a specific inhibitor of ULK1 to suppress HCC progression. However
Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro.
Liu X Y, et al.
Molecular Medicine Reports, 14(4), 3669-3675 (2016)
Enhancement of metastatic potential by γ-interferon.
Kelly S A, et al.
Cancer Research, 51(15), 4020-4027 (1991)
Differential effect of activin on mouse embryonic stem cell differentiation in insulin?secreting cells under nestin?positive selection and spontaneous differentiation protocols.
Jafary H, et al.
Cell Biology International, 32(2), 278-286 (2008)

Articles

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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