F4125
Ficin from fig tree latex
saline suspension, ≥1.0 units/mg protein (biuret)
Synonym(s):
Debricin, Ficain, higueroxyl delabarre
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
fig tree (latex)
Quality Level
form
saline suspension
specific activity
≥1.0 units/mg protein (biuret)
mol wt
23.8 kDa
storage temp.
2-8°C
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General description
Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Application
Ficin from fig tree latex has been used:
- in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
- for the digestion of eye tissue sections prior to immunostaining and immunofluorescence
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).
Physical form
Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0
Preparation Note
2× Crystallized
Inhibitor
Product No.
Description
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Substrate
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Certificates of Analysis (COA)
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Protocols
This procedure may be used for all Ficin products.
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