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F4639

Sigma-Aldrich

Monoclonal Anti-Fibrinogen antibody produced in mouse

clone FG-21, ascites fluid

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MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FG-21, monoclonal

contains

15 mM sodium azide

species reactivity

human, baboon

technique(s)

indirect ELISA: 1:4,000

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
F6140F9902F5766
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

Gene Information

human ... FGA(2243), FGB(2244), FGG(2266)

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Gene Information

human ... FGA(2243), FGB(2244), FGG(2266)

Gene Information

-

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

FG-21, monoclonal

clone

FN-3E2, monoclonal

clone

85D4, monoclonal

clone

VP-52, monoclonal

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General description

Fibrinogen, is a thrombin-coagulable soluble plasma 340kDa glycoprotein, composed of paired sets of three subunits i.e. α, β, γ. Among three subunits, β and γ subunits contain one N-glycosylation site, which is occupied by a biantennary N-glycan. It contains three pairs of disulfide bonded chains called α, β, γ which further folded into four structural domains: the D, E, connector and the COOH-terminal region of the Aα chain.
Monoclonal Anti-Fibrinogen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody is specific for human fibrinogen and recognizes an epitope sensitive to reduction commonly used in immunoblotting assays. It also reacts with synthetic human fibrinopeptides A and B, fibrinogen related peptides and baboon fibrinogen. No cross-reactivity with bovine, pig, sheep, horse, dog, goat, rabbit, guinea pig, or rat is observed.

Immunogen

purified haptoglobin from pooled human plasma containing the three major haptotypes.

Application

Monoclonal Anti-Fibrinogen antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • fibrinogen adsorption assay

Biochem/physiol Actions

Fibrinogen, a blood coagulation protein, is regarded as the central protein in the blood coagulation system.Fibrinogen plays a crucial role in protecting the vascular network against the loss of blood after tissue injury.
Plasmin attacks the Aα chain COOH domain to produce the heterogeneous fragment X. After a series of degradation, it ended with terminal digestion products: fragments D and E which represent the major globular domains in fibrinogen. Mutations in this gene leads to several disorders including hypofibrinogenemia, afibrinogenemia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Photon-modulated changes of cell attachments on poly (spiropyran-co-methyl methacrylate) membranes
Higuchi A, et al.
Biomacromolecules, 5(5), 1770-1774 (2004)
Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinoma
Li H, et al.
Proteome Science, 9(1), 21-21 (2011)
Preservation of hematopoietic stem and progenitor cells from umbilical cord blood stored in a surface derivatized with polymer nanosegments
Higuchi A, et al.
Biomacromolecules, 9(2), 634-639 (2008)
N E Kirschbaum et al.
The Journal of biological chemistry, 265(23), 13669-13676 (1990-08-15)
The COOH-terminal portion of the A alpha chain of human fibrinogen is highly susceptible to proteolytic degradation. This property has prevented isolation of the COOH-terminal domain of fibrinogen for the direct investigation of its functional characteristics. Human fibrinogen was degraded
Emmanuel Levrat et al.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 22(2), 148-150 (2011-01-20)
Congenital afibrinogenemia is a rare autosomal recessive coagulation disorder characterized essentially by bleeding symptoms, but miscarriages and, paradoxically, thromboembolic events can also occur. Most reported mutations leading to congenital afibrinogenemia are located in FGA encoding the fibrinogen A α-chain. In

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