MilliporeSigma
All Photos(4)

Documents

FD2000S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 2,000,000

Sign Into View Organizational & Contract Pricing

Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

biological source

synthetic (organic)

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 2,000,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to orange

solubility

H2O: soluble 25 mg/mL

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
FD150SFD40SFD250S
form

powder

form

powder

form

powder

form

powder

color

yellow to orange

color

faint yellow to orange

color

yellow to dark orange

color

yellow to orange

solubility

H2O: soluble 25 mg/mL

solubility

H2O: 25 mg/mL, clear to slightly hazy, yellow-orange

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

solubility

water: 25 mg/mL, clear, yellow

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
Fluorescein-dextran, 2000 kDa, was used for in vivo imaging of brain microvasculature in active mice using a minature integrated fluorecent microscope. FITC-dextran, 2000 kDa (FD-2000) is used in cardiovascular, microcirculation, perfusion and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Fluorescein-dextran, 2000 kDa, may be used for in vivo imaging of brain microvasculature in active mice using a miniature integrated fluorescent microscope. FITC-dextran has been used to study plant cell wall porosity.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Poornima Venkat et al.
Neurobiology of aging, 50, 96-106 (2016-12-13)
We investigated cognitive function, axonal/white matter (WM) changes and glymphatic function of vascular dementia using a multiple microinfarction (MMI) model in retired breeder (RB) rats. The MMI model induces significant (p < 0.05) cognitive decline that worsens with age starting
Haruna Hirata et al.
Biological & pharmaceutical bulletin, 37(2), 262-267 (2014-02-05)
Long-term peritoneal dialysis (PD) frequently produces morphological and functional changes of the peritoneum, which makes continuation of PD difficult. Moreover, the progression of peritoneal injury causes complications and poor prognosis. Since therapeutic treatments for peritoneal injury during PD have yet
Weiguo Chen et al.
American journal of respiratory cell and molecular biology, 50(2), 328-336 (2013-09-14)
The statins are now recognized to have pleiotropic properties, including augmentation of endothelial barrier function. To explore the mechanisms involved, we investigated the effect of simvastatin on endothelial cell (EC) tight junctions. Western blotting of human pulmonary artery ECs treated
Baoqiang Li et al.
PloS one, 14(8), e0221547-e0221547 (2019-08-31)
Chronic atherosclerosis may cause cerebral hypoperfusion and inadequate brain oxygenation, contributing to the progression of cognitive decline. In this study, we exploited two-photon phosphorescence lifetime microscopy to measure the absolute partial pressure of oxygen (PO2) in cortical tissue in both
Kunal K Ghosh et al.
Nature methods, 8(10), 871-878 (2011-09-13)
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal

Related Content

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service