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G3375

Sigma-Aldrich

Gliadin from wheat

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

wheat

Quality Level

form

powder

technique(s)

enzyme immunoassay: suitable
flow cytometry: suitable

UniProt accession no.

InChI

1S/C29H41N7O9/c30-18(15-16-5-7-17(37)8-6-16)27(42)35-13-1-3-21(35)25(40)33-19(9-11-23(31)38)28(43)36-14-2-4-22(36)26(41)34-20(29(44)45)10-12-24(32)39/h5-8,18-22,37H,1-4,9-15,30H2,(H2,31,38)(H2,32,39)(H,33,40)(H,34,41)(H,44,45)

InChI key

HZWWPUTXBJEENE-UHFFFAOYSA-N

Gene Information

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General description

Gluten consists of two families of prolamins, known as gliadin and glutenin. Gliadin is a strongly hydrophobic glycoprotein, with a very poor solubility. The gliadins can be further subdivided into α-gliadins, γ-gliadins and ω-gliadins (based on their order of mobility on electrophoresis at low pH).

Application

Gliadin from wheat has been used in
  • peptic tryptic digestion to study the effect of in vitro gliadin challenge on biopsies from celiac disease (CD) patients on gluten-free diet (GFD)
  • to prepare gliadin for antigliadin antibody assay
  • as a standard for the quantification of gliadin levels to the picogram level by flow cytometry

Biochem/physiol Actions

The gliadins comprise a family of glutamine- and proline-rich monomeric proteins that account for 40-50% of the total protein content of wheat kernels. The alcohol-soluble gliadins aggregate with the alcohol-insoluble glutenins to form gluten, which is responsible for the structure and properties of wheat dough. The gliadins are also involved in the pathogenesis of celiac disease. an autoimmune disease in which human leukocyte antigens DQ2 and DQ8 present gliaden peptides to a subset of T cells inducing the release of proinflammatory cytokines that, in turn, induce degradation of the intestinal mucosal matrix and cell death. B cells are also stimulated to produce gluten-specific and transglutaminase 2 (TG2)-specific antibodies, the latter may result from the presentation of gliadin peptides covalently bound to TG2.

Quality

Crude; From Wheat Gluten

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Jean-Baptiste Domergue et al.
International journal of molecular sciences, 23(9) (2022-05-15)
Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ15N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ15N analysis
Quantification of gliadin levels to the picogram level by flow cytometry
Capparelli R, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 63A(2), 108-113 (2005)
L J Chartrand et al.
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, 157(5), 527-533 (1997-09-19)
To test the effectiveness of serologic antigliadin antibody (AGA) testing in predicting celiac disease in children. Prospective clinical assessment. Hôpital Sainte-Justine, montreal. A total of 176 children with possible celiac disease who were referred for duodenal biopsy between January 1992
Sanja Petronijevic et al.
European journal of oral sciences, 128(1), 27-36 (2020-02-23)
Previously we detected increased levels of IgA to the enamel matrix protein amelogenin in children with untreated coeliac disease (CeD). The biological impact of autoantibodies to amelogenin may depend on which part of the amelogenin (epitopes) they bind. Sera or
K Sivasegaran et al.
International journal of cosmetic science, 29(2), 103-110 (2008-05-21)
Pre-screening of cosmetic ingredients is vital for consumer safety. Previous in vivo techniques, such as the Draize test, have proved to be unreliable in predicting ocular irritancy and therefore there is a need for alternate testing methodologies. One such test

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