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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

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Synonym(s):
blocking buffer
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

form

powder blend

storage temp.

room temp

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This Item
B6429GBL105101W0138
Sigma-Aldrich

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G7663

Gelatin blocking buffer

Sigma-Aldrich

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B6429

Casein Blocking Buffer 10x

Grace Bio-Labs Super G blocking buffer volume 500 mL, sterile

GBL105101

Grace Bio-Labs Super G blocking buffer

Sigma-Aldrich

Sigma-Aldrich

W0138

Western Blocker™ Solution

form

powder blend

form

solution

form

liquid

form

-

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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A Fortin et al.
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K A Brogden et al.
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Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In
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Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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