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Protein A Sepharose Cl-4B

Cytiva 17-0963-02, pack of 500 mL

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native protein A

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 500 mL


Cytiva 17-0963-02

storage condition

(20% Ehtanol)


4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm


2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range



~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

storage temp.


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General description

Protein A Sepharose CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.

Features and Benefits

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

Analysis Note

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Legal Information

Sepharose is a trademark of Cytiva






Storage Class

3 - Flammable liquids

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