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H4409

SAFC

EX-CELL® CD Hybridoma Medium, Chemically Defined

Animal-component free, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for hybridoma, suitable for cell culture

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Synonym(s):
Hybridoma Medium
MDL number:
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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This Item
H428114610C14621C
SAFC

SAFC

H4281

EX-CELL® Hybridoma Medium

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

components

HEPES: yes
NaHCO3: yes
L-glutamine: no
phenol red: no
glucose: 2.9 g/L

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

components

NaHCO3: 1.6 g/L
HEPES: 7.5 mM
L-glutamine: no
glucose: 3.45 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Features and Benefits

This medium offers the advantages of reduced raw material costs and uniform lot-to-lot product consistency. In addition, it reduces the risk of contamination by adventitious agents associated with animal-derived materials, making it of particular interest for biopharmaceutical applications. Cells can be successfully transferred from serum-supplemented cultures to these medium with little or no adaptation.
Developed to meet the needs of biotechnology manufacturing, this medium supports rapid initial cell growth and high levels of antibody expression. It also supports high cell densities at high viability for extended periods without the use of animal-derived proteins.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.

Reconstitution

Prior to use medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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