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H6648

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
HBSS
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

shipped in

ambient

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This Item
55021CH9394H8264
vibrant-m

H6648

Hanks′ Balanced Salt solution

vibrant-m

55021C

Hanks′ Balanced Salt solution

vibrant-m

H9394

Hanks′ Balanced Salt solution

vibrant-m

H8264

Hanks′ Balanced Salt solution

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

impurities

endotoxin, tested

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Application

Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
  • For assessing airway inflammation in mice

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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MMYOMAG-74K-13

1000309185

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Gentamicin solution 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

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Gentamicin solution

A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material
N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
Janhavi Sharma et al.
Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)
One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase
Iliana Moreschi et al.
The Journal of biological chemistry, 281(42), 31419-31429 (2006-08-24)
Micromolar concentrations of extracellular beta-NAD+ (NAD(e)+) activate human granulocytes (superoxide and NO generation and chemotaxis) by triggering: (i) overproduction of cAMP, (ii) activation of protein kinase A, (iii) stimulation of ADP-ribosyl cyclase and overproduction of cyclic ADP-ribose (cADPR), a universal
James W Voltz et al.
American journal of respiratory cell and molecular biology, 39(1), 45-52 (2008-02-16)
The roles of sex hormones as modulators of lung function and disease have received significant attention as differential sex responses to various lung insults have been recently reported. The present study used a bleomycin-induced pulmonary fibrosis model in C57BL/6 mice

Protocols

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Isolated from adult human heart ventricles, human cardiac fibroblasts (HCF) are used to study myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. They play a critical role in extracellular matrix maintenance, growth factor synthesis, and cytokine synthesis. This protocol outlines the recommended procedure for culturing and subculturing human cardiac fibroblasts.

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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