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I1643

Sigma-Aldrich

Pyrophosphatase, Inorganic from baker's yeast (S. cerevisiae)

powder, ≥500 units/mg protein (E1%/280)

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Synonym(s):
Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

powder

Quality Level

specific activity

≥500 units/mg protein (E1%/280)

mol wt

71 kDa

storage temp.

−20°C

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1 of 4

This Item
P7634P5381E6126
form

powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

71 kDa

mol wt

-

mol wt

tetramer 119,500 Da±600

mol wt

93 kDa

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

General description

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
  • as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
  • as a component of PCR mixture.
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

Physical form

Lyophilized powder containing 80-90% buffer salts

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Virginia P Ronchi et al.
The Journal of biological chemistry, 292(44), 18006-18023 (2017-09-20)
To understand the mechanism for assembly of Lys
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
Vuokko Jansson et al.
Analytical biochemistry, 304(1), 135-137 (2002-04-24)
Laura Pedró-Rosa et al.
Journal of biomolecular screening, 20(1), 122-130 (2014-08-29)
Improved therapies for the treatment of Trypanosoma brucei, the etiological agent of the neglected tropical disease human African trypanosomiasis, are urgently needed. We targeted T. brucei methionyl-tRNA synthetase (MetRS), an aminoacyl-tRNA synthase (aaRS), which is considered an important drug target
Jin He et al.
Annals of neurology, 93(2), 244-256 (2022-09-12)
Despite the increasing number of genes associated with Charcot-Marie-Tooth (CMT) disease, many patients currently still lack appropriate genetic diagnosis for this disease. Autosomal dominant mutations in aminoacyl-tRNA synthetases (ARSs) have been implicated in CMT. Here, we describe causal missense mutations
Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.
Roberto A Gaxiola et al.
Plant physiology, 159(1), 3-11 (2012-03-22)

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