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L2879

Sigma-Aldrich

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

Synonym(s):

Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

chicken egg white

Quality Level

type

Grade VI

form

lyophilized powder

specific activity

≥35,000 units/mg protein (E1%/282)

mol wt

~14.3 kDa

composition

Protein, ≥80%

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

cell analysis

storage temp.

−20°C

General description

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Application

Lysozyme chloride form from chicken egg white has been used:
  • as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
  • in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
  • in viscometric assay for determining lysozyme activity    

Biochem/physiol Actions

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder containing sodium chloride and sodium acetate

Preparation Note

3× Crystallized

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Sobrino-López et al.
Journal of dairy science, 91(5), 1759-1768 (2008-04-19)
High-intensity pulsed electric field (HIPEF) is a nonthermal treatment studied for its wide antimicrobial spectrum on liquid food, including milk and dairy products. Moreover, the antimicrobial effect of HIPEF may be enhanced by combining HIPEF with other treatments as hurdles.
Lysozyme-occurrence in nature, biological properties and possible applications
Gajda E and Bugla-P G
Journal of biological and chemical chronicles, 68, 1501-1515 (2014)
The effect of lysozyme and ethylenediaminetetraacetic acid on Salmonella on broiler parts
Samuelson, KJ, et al.
Poultry Science, 64(8), 1488-1490 (1985)
Viscometric assay for determining lysozyme activity
Yamaoka K, et al.
Analytica Chimica Acta, 219, 123-126 (1989)
Serum Lysozyme (Muramidase) Levels in Intra-Abdominal Abscesses: An Experimental Study
Daniel MP, et al.
The Indian Journal of Surgery (2015)

Protocols

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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