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L5520

Sigma-Aldrich

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Leibovitz L15
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

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form

liquid

form

powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

components

L-glutamine: no
glucose: high
phenol red: yes
sodium pyruvate: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Reconstitution

Supplement with 0.3 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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L-15 Media Formulation

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