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L6876

Sigma-Aldrich

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

form

lyophilized powder

specific activity

≥40,000 units/mg protein

mol wt

single-chain 14.3 kDa

composition

protein, ≥90%

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

chicken ... LYZ(396218)

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1 of 4

This Item
L4631L7773L3790
specific activity

≥40,000 units/mg protein

specific activity

-

specific activity

-

specific activity

-

mol wt

single-chain 14.3 kDa

mol wt

single-chain 14.3 kDa

mol wt

single-chain 14.3 kDa

mol wt

single-chain 14.3 kDa

UniProt accession no.

P00698

UniProt accession no.

P00698

UniProt accession no.

P00698

UniProt accession no.

P00698

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Application

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Features and Benefits

Lysozyme from chicken egg white is a versatile enzyme with unique properties that make it effective for various applications, including bacterial cell wall breakdown, genomic DNA extraction, and external standard for mass analysis. It is reliable, active over a broad pH range, and available in various packaging sizes.

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Inhibition of lung tumor colonization and cell migration with the disintegrin crotatroxin 2 isolated from the venom of Crotalus atrox.
Galan JA et al.
Toxicon, 51, 1186-1186 (2008)
Valerio Iebba et al.
Scientific reports, 8(1), 8210-8210 (2018-05-31)
In liver cirrhosis (LC), impaired intestinal functions lead to dysbiosis and possible bacterial translocation (BT). Bacteria or their byproducts within the bloodstream can thus play a role in systemic inflammation and hepatic encephalopathy (HE). We combined 16S sequencing, NMR metabolomics
Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
Chen W et al.
PLoS ONE, 7, e39743-e39743 (2012)
Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms.
Caufield PW and Walker TM
Journal of Clinical Microbiology, 27, 274-274 (1989)
Sara Hernández-Pérez et al.
Journal of cell science, 133(5) (2019-11-30)
In order to mount high-affinity antibody responses, B cells internalise specific antigens and process them into peptides loaded onto MHCII for presentation to T helper cells (TH cells). While the biochemical principles of antigen processing and MHCII loading have been

Articles

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Protocols

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Chromatograms

application for HPLC

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