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MAK003

Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

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usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; neurological disorders

storage temp.

−20°C

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MAK003

Branched Chain Amino Acid Kit

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MAK352

DL-Serine Assay Kit

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MAK004

Glutamate Assay Kit

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 fluorometric tests

usage

sufficient for 100 colorimetric tests

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; neurological disorders

relevant disease(s)

cardiovascular diseases

relevant disease(s)

aging/geriatric diseases; neurological disorders

relevant disease(s)

neurological disorders; cancer

General description

The branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals. Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies, and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.

Application

Branched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.

Suitability

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological samples.

Principle

In this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Protein substitution to produce a processed cheese with high branched-chain amino acids of medical and genetic importance.
El-Shazly H A M, et al.
The Egyptian journal of medical human genetics., 11(2), 121-133 (2010)
Multifaceted role of insulin-like growth factors and mammalian target of rapamycin in skeletal muscle.
Frost R A and Charles H L
Endocrinol. Metab. Clin., 41(2), 297-322 (2012)
Itziar Lamiquiz-Moneo et al.
Cells, 9(3) (2020-03-18)
The global prevalence of type 2 diabetes (T2D) is increasing rapidly, especially in low- and middle-income countries and has a high number of associated comorbidities. Plasmatic concentrations of branched chain amino acids (BCAA) and retinol-binding protein 4 (RBP4) have been
Aidan M Sokolov et al.
Human molecular genetics, 29(18), 3003-3013 (2020-08-22)
Pathogenic mutations in the solute carrier family 7 member 5 (SLC7A5) gene, which encodes an amino acid transporter cause microcephaly and seizures, yet the mechanisms responsible for these phenotypes are unclear. Models have demonstrated that Slc7a5 deletion is embryonic lethal
Hager M Kowash et al.
Clinical science (London, England : 1979), 136(15), 1117-1137 (2022-07-20)
Maternal infection during pregnancy increases the offspring risk of developing a variety of neurodevelopmental disorders (NDDs), including schizophrenia. While the mechanisms remain unclear, dysregulation of placental function is implicated. We hypothesised that maternal infection, leading to maternal immune activation and

Articles

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

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