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MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

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Synonym(s):
Green PKH membrane mini labeling kit
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

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This Item
PKH67GLMINI26MIDI67
packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

manufacturer/tradename

-

manufacturer/tradename

Distributed for Phanos Technologies

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

application(s)

cell analysis
detection

application(s)

-

application(s)

cell analysis
detection

application(s)

cell analysis
detection

General description

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Application

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description

  • Diluent C 10 mL

  • PKH67 Cell Linker in ethanol 0.1 mL

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

55.4 °F - closed cup

flash_point_c

13 °C - closed cup


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Articles

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service