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N2876

Sigma-Aldrich

Neuraminidase from Clostridium perfringens (C. welchii)

Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder

Synonym(s):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

type

Type V

form

lyophilized powder

specific activity

≥0.1 units/mg solid (using mucin)
≥1.3 units/mg solid (using 4MU-NANA)

application(s)

diagnostic assay manufacturing

foreign activity

Protease and NAN-aldolase, present

shipped in

dry ice

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

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General description

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Application

Neuraminidase from Clostridium perfringens has been used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase.

Biochem/physiol Actions

Neuraminidase can increase aggregation in certain cell lines by removing exposed negatively charged sialic acid residues on the cell surface.
Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.
Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.
The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Unit Definition

One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin.

One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)

Preparation Note

Prepared by salt fractionation.

Analysis Note

Package sizes based on 4MU-NANA units
Package sizes based on the 4MU-NANA units

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes.
M S Weiner et al.
Blood, 42(6), 939-946 (1973-12-01)
Randa Bittar et al.
Practical laboratory medicine, 18, e00150-e00150 (2020-01-08)
A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method
J J Deman et al.
The Journal of cell biology, 60(3), 641-652 (1974-03-01)
Aggregation of suspended HeLa cells is increased on removal of cell surface sialic acid. Calcium ions promote aggregation whereas magnesium ions have no effect. The calcium effect is abolished by previous treatment of the cells with neuraminidase. Trypsinization of the
S Gatt et al.
The Biochemical journal, 193(1), 267-273 (1981-01-01)
Studies were done on the effect of bile salts on the rates of hydrolysis of the N-acetylneuraminyl linkages of several sialic acid-containing compounds by the neuraminidase of Clostridium perfringens. When GM3-ganglioside, two glycolipids (glycophorin and orosomucoid) and neuraminyl-lactose were used
Francesca Ferrara et al.
Frontiers in immunology, 12, 661379-661379 (2021-06-11)
Influenza B viruses (IBV) cause respiratory disease epidemics in humans and are therefore components of seasonal influenza vaccines. Serological methods are employed to evaluate vaccine immunogenicity prior to licensure. However, classical methods to assess influenza vaccine immunogenicity such as the

Articles

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Protocols

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

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