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N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Ham’s F-12
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
N6658N8641FG0815
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes
phenol red: yes
HEPES: yes

components

stable glutamine: yes
HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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