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N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

Product Name

Nutrient Mixture F-12 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL6335
RNBL4635
RNBL0455
RNBK7756
RNBK3282

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