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N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

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Synonym(s):
Neutralizing Buffer for Blotting Techniques
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

powder

technique(s)

Southern blotting: suitable

foreign activity

DNase, RNase, none detected

storage temp.

room temp

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This Item
S8140S6639G2526
Sigma-Aldrich

Sigma-Aldrich

N6019

Neutralizing Buffer

Sigma-Aldrich

Sigma-Aldrich

S8140

SSPE Buffer

Gel Loading Buffer for NA electrophoresis

Sigma-Aldrich

G2526

Gel Loading Buffer

form

powder

form

powder blend

form

solution

form

liquid

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

technique(s)

-

foreign activity

DNase, RNase, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

RNAse, none detected

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

Application

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

Components

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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