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N6760

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
Ham’s F-12
MDL number:
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: yes
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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This Item
F6636N8641N3520
SAFC

SAFC

F6636

Nutrient Mixture F-12 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: yes
NaHCO3: no

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

components

L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes
phenol red: yes
HEPES: yes

components

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Low serum media

hybrid cell cultue, amino acid enriched

-

differentiated cell culture medium, primary human liver cell cultuer medium

Application

Developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells.

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.176 g/L sodium bicarbonate.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Muath Helal et al.
Frontiers in microbiology, 11, 147-147 (2020-03-03)
Invasive fungal infections (IFI) cause devastating morbidity and mortality, with the number of IFIs more than tripling since 1979. Our laboratories were the first to demonstrate that radiolabeled microorganism-specific monoclonal antibodies are highly effective for treatment of experimental fungal, bacterial
Claudia Maria Bertan Membrive et al.
Journal of animal science and biotechnology, 5(1), 25-25 (2014-05-30)
Estradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore (CI)
Susana P Mejía et al.
Frontiers in pharmacology, 12, 685391-685391 (2021-07-16)
Infectious diseases caused by intracellular microorganisms represent a significant challenge in medical care due to interactions among drugs during coinfections and the development of resistance in microorganisms, limiting existing therapies. This work reports on itraconazole (ITZ) encapsulated into functional polymeric
Rani Ojha et al.
The Journal of urology, 191(6), 1889-1898 (2014-01-21)
We evaluated the status of autophagy in different grades of urothelial carcinoma and explored autophagy modulators as a potential adjunctive therapeutic agent for urothelial carcinoma. The study was performed in tumor tissue from patients with low and high grade urothelial
SunilGowda Sunnaghatta Nagaraja et al.
Toxicology in vitro : an international journal published in association with BIBRA, 70, 105037-105037 (2020-11-06)
Lung cancer patients who have undergone radiotherapy developed severe complications such as pneumonitis and fibrosis. Upon irradiation, epithelial cells acquire mesenchymal phenotype via a process called epithelial to mesenchymal transition (EMT), which plays a vital role in organ fibrosis. Several

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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