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N8271

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
9001-67-6
Enzyme Commission number:
3.2.1.18 (BRENDA, IUBMB)
MDL number:
MFCD00131711
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

form

buffered aqueous solution

specific activity

≥135 units/mg protein

mol wt

88 kDa
 95 kDa

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

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Related Categories

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Alana L Woodward et al.
Veterinary microbiology, 169(3-4), 113-127 (2014-02-01)
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during 2010-2012, including in vaccinated animals, highlighting the importance of surveillance and virus characterisation. Virus isolates
Chinh Tran-To Su et al.
BMC bioinformatics, 14 Suppl 16, S7-S7 (2014-02-26)
Since late March 2013, there has been another global health concern with a sudden wave of flu infections by a novel strain of avian influenza A (H7N9) virus in China. To-date, there have been more than 100 infections with 23
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
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