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P3563

Sigma-Aldrich

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4

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Synonym(s):
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
eCl@ss:
32129211
NACRES:
NA.25

form

powder

Quality Level

solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

cell analysis
diagnostic assay manufacturing

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This Item
P3813P3744P4244
solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

solubility

water: soluble

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

General description

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Application

Phosphate buffered saline has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
  • cell isolation

Packaging

Foil pouches

Reconstitution

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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