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P5380

Sigma-Aldrich

Protease from Bacillus licheniformis

Type VIII, lyophilized powder, 7-15 units/mg solid

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Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Bacillus licheniformis

Quality Level

type

Type VIII

form

lyophilized powder

specific activity

7-15 units/mg solid

mol wt

27 kDa

purified by

crystallization

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless

foreign activity

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

storage temp.

−20°C

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1 of 4

This Item
P4860P8038P5459
Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Sigma-Aldrich

P8038

Proteinase, bacterial

form

lyophilized powder

form

aqueous solution

form

lyophilized powder

form

glycerol solution (50%)

specific activity

7-15 units/mg solid

specific activity

≥2.4 U/g

specific activity

≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid

specific activity

≥7 units/mg protein (biuret)

mol wt

27 kDa

mol wt

-

mol wt

27 kDa

mol wt

-

purified by

crystallization

purified by

-

purified by

crystallization

purified by

-

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

solubility

-

solubility

-

solubility

0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible

General description

The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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