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P5472

Sigma-Aldrich

Precast Agarose Gels

8-well (portrait orientation)

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Synonym(s):
Precast agarose gels for high resolution nucleic acids separation
NACRES:
NB.22

grade

for molecular biology

Quality Level

form

gel form

contains

0.5 μg/mL ethidium bromide

storage temp.

room temp

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This Item
P6222GE17-0554-0211696505001
Precast Agarose Gels 8-well (portrait orientation)

Sigma-Aldrich

P5472

Precast Agarose Gels

Precast Agarose Gels 8-well (portrait orientation)

Sigma-Aldrich

P6222

Precast Agarose Gels

Agarose NA Cytiva 17-0554-02, pack of 100 g

GE17-0554-02

Agarose NA

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

form

gel form

form

gel form

form

solid

form

-

contains

0.5 μg/mL ethidium bromide

contains

-

contains

-

contains

-

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

100

General description

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style

Application

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 500 - 1000 base pairs.

Packaging

Package of 20 individually foil-wrapped gels.

Components

Precast gels contain 1.0% agarose in 1X TBE buffer with 0.5 ug/ml ethidium bromide.

Other Notes

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Quality

No detected DNase or RNase activity

Physical form

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Precast Agarose Gels

These gels are suitable for separating nucleic acids, giving sharp DNA bands and low background fluorescence.

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