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RAB0352

Sigma-Aldrich

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

192 assays

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NACRES:
NA.32

usage

192 assays

species reactivity

mouse, human, rat

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABBLOCK5x Blocking Solution (Item F)SDS

  • RABFIX1Fixing Solution (Item D)SDS

  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS

  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS

  • RABPLATE1Uncoated MicroplateSDS

  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS

  • RABSTOP1Stop SolutionSDS

  • RABTMB1TMB Substrate Reagent (Item J)SDS

  • RABWASH120X Wash Buffer Concentrate ASDS

  • RABWASH220X Wash Buffer Concentrate BSDS

See All (10)

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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