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RAB0477

Sigma-Aldrich

Mouse Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

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Synonym(s):
Cachectin, TNF-alpha, Tumor necrosis factor alpha
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Tnf(21926)

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RAB0340RAB0478RAB0522
species reactivity

mouse

species reactivity

human

species reactivity

porcine

species reactivity

bovine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 74.07-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 0.014-10 ng/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.1 ng/mL

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.

Immunogen

Recombinant Mouse TNF-α

Application

Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.

Biochem/physiol Actions

Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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25G
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Ding LZ, et al.
International Journal of Molecular Medicine, 41(5), 2517-2526 (2018)
Tumor necrosis factor alpha: a link between neuroinflammation and excitotoxicity
Olmos G and Llado J
Mediators of Inflammation (2014)
Pharmacokinetic and pharmacodynamic characterisation of an anti-mouse TNF receptor 1 domain antibody formatted for in vivo half-life extension
Goodall LJ, et al.
PLoS ONE, 10(9), e0137065-e0137065 (2015)
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Tan S, et al.
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Ying Wang et al.
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