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Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

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Synonym(s):
20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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This Item
SRE0068S0902P7059
Sigma-Aldrich

Sigma-Aldrich

S0902

SSC Buffer

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

0.2 μm filtered

form

solution

form

liquid

form

powder blend

form

liquid

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

500

Quality Level

200

Quality Level

200

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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