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3dGRO® Spheroid Medium

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CSC media, Cancer stem cell media, tumorsphere culture media, tumorsphere media


pkg of 250 mL (basal medium)

shipped in

wet ice

storage temp.


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General description

Cancer stem cell (CSCs) are defined as a small subset of cells within a tumor with the ability to self-renew and often drive tumor progression and recurrence after chemotherapy treatment. Therefore, development of specific therapies targeted at CSCs holds hope for improvement of survival and quality of life of cancer patients, especially for patients with metastatic disease.
Traditionally, cancer stem cells have been isolated from cancer cell lines and tumor biopsies and grown in undefined serum containing media using 3-dimensional cell cultures. Using these undefined culture conditions leads to experimental variability and suboptimal cell growth.
3dGRO® Spheroid Medium is a new chemically defined and serum-free cancer stem cell media that supports the expansion of CSC′s in 3D spheroid cell cultures. The media supports serial passaging of cancer cell lines leading to an overall enrichment of CSC populations.

Features and Benefits

  • Chemically defined, Serum-free formulation.
  • High tumorsphere formation efficiency.
  • Allows extended serial tumorsphere passaging (>10).
  • Proven to enrich CSCs from numerous cancer cell lines.

Storage and Stability

Upon receipt, the basal medium can be stored at 4° C and the vial of supplement can be stored at -20° C stably until the expiration date.

Before first use, the the entire contents of the supplement vial should be added to the basal medium. The shelf life of the complete medium after addition of the supplement is 6 weeks when stored at 4° C. It is recommended to aliquot and prewarm only the required amount of complete medium for each use and to continue to store the remainder at 4° C.

Other Notes

Cell Lines tested with 3dGRO® Spheroid Medium:
MCF-7, A-431, Panc-1, LNCaP, HT-29, A-549, U-87 MG, HT1080, E006AA

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids



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Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.


Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

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