S4920
SITE Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Media supplement
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About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
General description
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
Application
SITE Liquid Media Supplement (100×) has been used:
- in the 3D culture of mouse embryonic pancreatic epithelial cells
- to isolate rabbit gastric glands and parietal cells
- to isolate rabbit single proximal tubule cells
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element normally provided by serum.
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
Components
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Proceedings of the National Academy of Sciences of the United States of America, 79(4), 1158-1162 (1982-02-01)
A serum-free medium supplemented with a few growth factors was devised to grow lymphocyte hybridomas. The medium was developed with the hybridoma line MPC11-BL, a fusion product between a mouse plasmacytoma cell line (MPC11TG70na3) and mouse (BALB/c) spleen cells. In
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