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T8158

Sigma-Aldrich

Transferrin human

powder, BioReagent, suitable for cell culture

Synonym(s):
Siderophilin, partially saturated
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

product line

BioReagent

Quality Level

Assay

≥98%

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested
hepatitis and HIV antibody, none detected

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... TF(7018)

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This Item
T3705T2036T3309
Sigma-Aldrich

Sigma-Aldrich

T8158

Transferrin human

Transferrin human recombinant, expressed in rice

Sigma-Aldrich

T3705

Transferrin human

apo-Transferrin human powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

T2036

apo-Transferrin human

Sigma-Aldrich

Sigma-Aldrich

T3309

Transferrin human

technique(s)

cell culture | mammalian: suitable

technique(s)

immunoprecipitation (IP): suitable, tissue culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

impurities

endotoxin, tested, hepatitis and HIV antibody, none detected

impurities

≤1.0 EU/mg Endotoxin

impurities

HIV and HBsAg, source material tested negative, endotoxin, tested

impurities

≤1.0 EU/mg endotoxin

solubility

H2O: 50 mg/mL

solubility

-

solubility

H2O: 50 mg/mL

solubility

-

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:
  • to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
  • as a supplement in serum-containing/devoid medium for human thyroid cell culture
  • as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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Cells: Molecules and Mechanisms, 174-174 (2009)
A Dautry-Varsat et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(8), 2258-2262 (1983-04-01)
At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to

Articles

Transferrin in Cell Culture

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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