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TRUEENZ

Sigma-Aldrich

TrueGel3D Enzymatic Cell Recovery Solution

solution, 500 μl

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shipped in

dry ice

storage temp.

−70°C

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vibrant-m

TRUE6

TrueGel3D Hydrogel Kits

vibrant-m

TRUE3

TrueGel3D Hydrogel Kits

storage temp.

−70°C

storage temp.

−70°C

storage temp.

-

storage temp.

−70°C

General description

TrueGel3D enzymatic cell recovery solution is extracted from Chaetomium gracile, which cleave glycosidic bonds of dextran polymers. It is used to dissolve dextran polymer to recover chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

TrueGel3D enzymatic cell recovery solution is used to dissolve dextran-based hydrogels to recover cells for post-culture analysis

Components

TRUEENZ-500UL contains TrueGel3D enzymatic cell recovery solution (dextranase)

Caution

Avoid loss of enzymatic activity by storing at -70°C

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

The TrueGel3D™ hydrogel is formed by crosslinking RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. Troubleshoot hydrogel formation and cell viability with this article.

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Protocols

A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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