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W1754

Sigma-Aldrich

Water

PCR Reagent

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Synonym(s):
H2O
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

PCR Reagent

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected
RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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W1754

Water

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vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

3 mmHg

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

PCR: suitable

technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Suitability

Suitable for polymerase chain reaction (PCR)

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
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Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
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Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
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Articles

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Protocols

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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