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Y1375

Millipore

YPD Broth

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Yeast Peptone Dextrose Broth
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

composition

Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

packaging

poly bottle of 1 kg
poly bottle of 250 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

color

beige

pH

6.3-6.7

application(s)

environmental
food and beverages

microbiology

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

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Millipore

Y1375

YPD Broth

Nutrient Broth Liquid medium used for the cultivation of a wide variety of microorganism, NutriSelect® Plus, suitable for microbiology

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Nutrient Broth

Elliker Broth suitable for microbiology, NutriSelect® Plus

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17123

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Millipore

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C1551

Czapek-Dox broth

form

powder

form

powder

form

powder

form

powder

composition

Bacteriological peptone, 20 g/L , Glucose, 20 g/L , Yeast extract, 10 g/L

composition

D(+)-glucose, 1 g/L , peptone, 15 g/L , sodium chloride, 6 g/L , yeast extract, 3 g/L

composition

ascorbic acid, 0.5 g/L , casein enzymic hydrolysate, 20 g/L , dextrose, 5 g/L , gelatine, 2.5 g/L , lactose, 5 g/L , saccharose, 5 g/L , sodiumacetate, 1.5 g/L , sodium chloride, 4 g/L , yeast extract, 5 g/L

composition

-

packaging

poly bottle of 1 kg, poly bottle of 250 g

packaging

-

packaging

-

packaging

-

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

General description

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Application

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Quantity

50 g per liter

Preparation Note

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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ClpS2 is a small protein under development as a probe for selectively recognizing N-terminal amino acids of N-degron peptide fragments. To understand the structural basis of ClpS2 specificity for an N-terminal amino acid, all atom molecular dynamics (MD) simulations were
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The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal cellulase, and fermentation
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Articles

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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