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ZMS1042

Sigma-Aldrich

Anti-ssDNA Antibody, clone 16-19 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Single stranded DNA

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

16-19, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

purified by

using protein G

species reactivity

bovine

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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clone

16-19, recombinant monoclonal

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conjugate

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antibody form

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antibody form

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antibody form

purified antibody

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 16-19 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects ssDNA.

Immunogen

Hybridoma generated by fusion of splenocytes from non-immunized BW and myeloma cell line Sp 2/0-Ag 14.

Application

Quality Control Testing

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

Tested applications

Enzyme Immunoassay Analysis (ELISA): Serial of dilutions from a representative lot detected ssDNA from Calf thymus.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ssDNA in Molt 4 cells treated with Etoposide (10 M; overnight).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

Target description

DNA, the carrier of genetic information, is a crucial part of basic biological research and synthetic biology. It exists as a double-helix structure and the two DNA strands known as polypeptides are composed of monomeric units known as nucleotides. Each nucleotide consists of four bases: adenine, guanine, cytosine, thymine and a deoxyribose sugar moiety. The nitrogenous bases of the two separate strands are bound together by base pairing (A-T and C-G) to create a double stranded DNA. Under certain conditions, these two strands can come apart (melting) to generate two single-stranded DNA. Single-stranded DNA (ssDNA) is a crucial DNA damage signal found at the sites of DNA damage that helps in recruitment of checkpoint kinases to the site of damage. This leads to cell cycle arrest, transcriptional upregulation of DNA repair genes, and modulation of DNA replication pathways. Hence, ssDNA is considered as a key intermediate in nearly all biochemical reactions involving DNA replication and DNA repair. However, the ssDNA structure is not stable and single-stranded DNA-binding proteins (SSB) hold the ssDNA intact and exposed during the course of its function, such as DNA transcription, recombination, and repair, and serve as template for opposite strand DNA synthesis. Antibodies against ssDNA are commonly used in ELISA-based test for the quantitative determination of IgG antibodies against ssDNA and have been used in serological detection of systemic lupus erythematosus (SLE) and drug induced lupus. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rekvig, OP. (2022). Front. Immunol. 12; 808008; Bantele, SCS., et al. (2019). Nat. Commun. 10; Article 944; Ballard, DW., and Voss EW. (1985). J. Immunol. 135(5); 3372-3380).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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