HPLC Analysis of Isocyanates on Titan™ C18
CONDITIONS
column
Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)
mobile phase
[A] 0.05% formic acid in 5:95 acetonitrile:water; [B] 0.05% formic acid in 95:5 acetonitrile:water
gradient
50% to 90% B in 1 min, hold at 90% B for 3 min, 90% to 50% B in 0.1 min, hold at 50% B for 3.9 min
flow rate
0.5 mL/min
pressure
3916 psi (270 bar)
column temp.
30 °C
detector
MS, ESI (+)
detector
MRM
injection
2 μL
sample
isocyanates in acetonitrile, 0.06 μg/mL
Description
Analysis Note
Analysis of DBA-derivatized isocyanate monomers on Titan C18 using LC-MS/MS.
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