TLC Analysis of Nucleoside Monophosphates on HPTLC Pre-coated Plate RP-18 WF 254s with Concentrating Zone
CONDITIONS
plate
HPTLC pre-coated plate RP-18 WF 254s with concentrating zone (1.13124.0001)
eluent
water containing 0.2M 1-butanesulfonic acid sodium salt
migration distance
5 cm
chamber
normal chamber without chamber saturation
sample
5 μL, 0.01%
detector
In-situ evaluation with TLC/HPTLC scanner (Camag) UV 260 nm