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TLC Analysis of Nucleoside Monophosphates on HPTLC Pre-coated Plate RP-18 WF 254s with Concentrating Zone

TLC Analysis of Nucleoside Monophosphates on HPTLC Pre-coated Plate RP-18 WF 254s with Concentrating Zone application for TLC

CONDITIONS

plate

HPTLC pre-coated plate RP-18 WF 254s with concentrating zone (1.13124.0001)

eluent

water containing 0.2M 1-butanesulfonic acid sodium salt

migration distance

5 cm

chamber

normal chamber without chamber saturation

sample

5 μL, 0.01%

detector

In-situ evaluation with TLC/HPTLC scanner (Camag) UV 260 nm