SRP4595

IFN-alpha 2b human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): IFNA, INFA2, Interferon α 2b
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: >2.0x10^7 units/mg
• mol wt: ~19.3 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: Q86UP4
• UniProt accession no.: Q86UP4
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IFNA2b(3440)

Description

General description

The gene IFN-α2b (interferon α 2b) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Human IFN-α2b is a 19.3kDa protein containing 165 amino acid residues. Structurally, IFN-α2b is O-glycosylated at the threonine residue at position 106.

Application

IFN-α2b human has been used:

• as an immunotherapeutic agent to test its antiproliferative and proapoptotic effects in RCC cell lines
• to stimulate peripheral blood mononuclear cell (PBMC) prior to RNA isolation
• to stimulate H4 cells before immunoprecipitation
• to study role of USP18 (ubiquitin specific peptidase 18)-mediation deISGylation in controlling cellular autophagy upon treatment with type I interferons

Biochem/physiol Actions

IFN-α2b (interferon α 2b) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2b also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene. IFN-α2b induces the secretion of cytokine IFN-γ in T cells. The hydrogels embedded with IFN-α2b exploits this functionality in gastric cancer cells. Recombinant IFN-α types are effective against Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in in vitro studies.

Physical form

Lyophilized from 5 mM sodium phosphate, pH 7.4.

Reconstitution

Reconstitute in water to a concentration of 1 mg/mL. This solution can then be diluted into other aqueous buffers. Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable