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SRP3058

Sigma-Aldrich

IFN-γ human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Immune Interferon, MAF, T cell interferon, type II interferon
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

5.0-10.0 ng/mL ED50

mol wt

16.7 kDa

packaging

pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

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vibrant-m

SRP3058

IFN-γ human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

mass spectrometry (MS): suitable

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

mol wt

16.7 kDa

mol wt

-

mol wt

calculated mol wt 17 kDa

mol wt

calculated mol wt 17 kDa

form

lyophilized

form

-

form

lyophilized powder

form

lyophilized powder

General description

IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. The gene is mapped to human chromosome 12q15. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.

Application

IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.

Biochem/physiol Actions

IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils. It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis. IFN-γ is also involved in Hepatitis E virus disease pathogenesis.

Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Perturbation of the T-cell repertoire in patients with unstable angina.
Liuzzo G
Circulation, 100, 2135-2139 (1999)
CD133+ liver cancer stem cells resist interferon-gamma-induced autophagy.
Li J
BMC Cancer, 16, 15-15 (2016)
Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells.
Szabo SJ
Science, 295, 338-342 (2002)
Dynamic regulation of IFN-gamma signaling in antigen-specific CD8+ T cells responding to infection.
Haring JS
Journal of Immunology, 174, 6791-6802 (2005)
MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines.
Dorner BG
Proceedings of the National Academy of Sciences of the USA, 99, 6181-6186 (2002)

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